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Objective To investigate the expression of human epididymal protein 4 (HE4) in chronic kidney disease (CKD) and its clinical value.Methods From April 2014 to December 2015,Serum samples of 92 non-cancer patients diagnosed as CKD and 84 healthy controls were collected in Nantong University Hospital.HE4,BUN,Scr,Cys C and β2MG were detected.The difference of HE4 expression in different stages of CKD and the correlation of HE4 with Urea,Scr,Cys C and β2MG were analysized,respectively.ROC curve was used to evaluate the auxiliary diagnostic value of HE4,BUN,Scr,Cys C and β2MG.Results The serum HE4 expression in patients with impaired renal function was 388.2 (130.1～1 659.5)mIu/ L,(F=16.237,P=0.001).Which was significantly higher than that in the control group [38.1 (32.77 ～ 48.17)mIu/L].The serum HE4 expression was increased by the stage of renal damage and the difference was existed among different groups (P＜0.05).HE4 expression was positive related with Cys C and β2MG.The AUC of HE4,BUN,Scr,Cys C and β2MG were 0.878,0.785,0.816,0.874 and 0.819,respectively.Conlusion It needs to consider the exsits of impaired renal function when the HE4 was detected.The HE4 might be a novel early diagnostic indication for CKD.
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Objective To observe the influence of emotional intervention on alcohol-induced mental disor-ders patients and provide guidance for health intervention.Methods 118 patients with alcohol -induced mental disorders were divided into emotional intervention group and conventional intervention group.After 4 weeks,the effect was evaluated by anxiety,depression,SDSS,BPRS,SCL-90.Results After 4 weeks intervention,the lost rate of follow up in the emotional intervention group was 3.57%,which in the conventional intervention group was 8.06%.In the emotional intervention group, the differential values before and after intervention of anxiety, depression, BPRS, SDSS,somatization,compulsion,interpersonal relationship sensitivity,depression dimension,anxiety dimension,rivalry, consternation,crankiness and spirit were (19.90 ±3.18),(23.02 ±4.26),(15.46 ±3.29),(1.89 ±0.91), (0.48 ±0.24),(0.29 ±0.22),(0.30 ±0.24),(0.26 ±0.24),(0.31 ±0.19),(0.16 ±0.17),(0.32 ±0.20), (0.36 ±0.29),(0.35 ±0.25) respectively.Compared with the conventional intervention group,the differential values were higher than conventional intervention group [(8.27 ±1.72),(12.31 ±2.64),(11.69 ±4.17), (1.52 ±0.84),(0.25 ±0.17),(0.12 ±0.14),(0.19 ±0.18),(0.18 ±0.16),(0.15 ±0.13),(0.09 ±0.07), (0.11 ±0.12),(0.17 ±0.14),(0.16 ±0.15)].There were significant differences between the two groups (anxiety:t=4.159,P=0.000;depression:t=16.013,P=0.000;BPRS:t=5.269,P=0.000;SDSS:t=2.227,P=0.028;somatization:t=5.851,P=0.000;compulsion:t=4.883,P=0.000;interpersonal relationship sensitivity:t=2.741,P=0.001;depression:t=2.076,P=0.004;anxiety:t=5.202,P=0.000;rivalry:t=2.864,P=0.005;consternation:t=6.749,P=0.000;crankiness:t=4.432,P=0.000;spirit:t =4.885,P=0.000).Conclusion Emotional intervention has positive effect on emotional states,somatic symptoms and quality of life,it is worthy of clin-ical promotion.
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Objective To investigate the expression and clinical value of miR-127-3p in plasma of patients with breast cancer .Methods 80 cases of breast patients , 70 cases of benign breast tumor patients and 70 cases of normal control group were recruited .A real-time fluorescent quantitative reverse transcription polymerase chain reaction ( RT-PCR ) method for detecting miR-127-3p was established; Liner, reproducibility, and specificity were evaluated.In addition, correlations between the relative expression of plasma miR-127-3p and the concentrations of CEA and CA 153 were assessed.the relationship of miR-127-3p expression and clinicopathological features was further determined by Mann-Whitney test.Results The method for detection of plasma miR-127-3p was established.The relative plasma expression of miR-127-3p in breast patients [ 10.561 ( 5.424 -16.465 ) ] was significantly higher than that in benign breast tumor patients [3.015 (1.987-5.035)] (P=0.000 6) and healthy controls [2.375 (1.173-4.370)] (P=0.000 2).However, there was no significant difference between benign tumor patients and healthy control group (P=0.143).Positive relationship was found between the relative expression of miR-127-3p and the concentration of CA153 (R2 =0.457, P=0.003).The area under the ROC curve (AUC) of miR-127-3pwas 0.763, which was higher than that of CEA and CA 153.No significant difference was found between plasma miR-127-3p expression and clinicalpathological features including tumor size , differentiation and tumor node metastasis stage (P>0.05).Conclusions miR-127-3p was increased in breast cancer patients and may be an important diagnostic index for breast cancer .
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Objective To prove the remarkable inhibitive effect of multiple siRNAs targeting a proliferation-inducing ligand (APRIL) on the human colorectal cancer cell.Methods We constructed a multiple short hairpin RNA(shRNA) expression vector containing four shRNAs (pG4) as well as four single one (pGsh644,pGsh1451,pGsh1938,pGsh2231) against APRIL gene in SW480 cell,and then transfected them into the human colorectal cancer cell line by cationic liposome.Ultimately,SW480 were screened by EGFP to obtain expression cell lines.APRIL expression levels including mRNA and APRIL protein were detected after transfected with all different kinds of vectors.Results A multiple shRNA expression vector containing four shRNAs (pG4) and four single ones were successfully constructed.Four single vectors (pGsh644,pGsh1451,pGsh1938,pGsh2231) and the multiple siRNAs expression vector (pG4) all decreased the APRIL mRNA by 56.2％,49.5％ ;50.9％,49.2％ and 79.3％.And APRIL protein expression was also remarkably reduced,especially by multiple siRNAs expression vector(87.5％).Conclusion Multiple siRNAs expression vector produced a more significant knockdown effect of APRIL than the vectors containing only one APRIL shRNA.What we found suggested us using the vector containing multiple shRNA to silence the expression of APRIL might be exploited as a novel therapeutic strategy for tumors.
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As is known in literature, the Base-scale Entropy Measure is useful for analyzing short-term heart rate variability signals. In this study, the method is applied to detect cardiac arrhythmias, including sinus and atrial arrhythmia. We detect and analyze normal sinus rhythm (NSR), atrial premature contraction (APC) and sinus bradycardia (SBR) signals. The selected data of NSR are obtained from the MIT-BIH normal sinus database, while others are from the MIT-BIH arrhythmia database. The results show the Base-scale Entropy can also detect sinus and atrial arrhythmia.